BIOASSAY OF DIFFERENT EXTRACT OF Clerodendrum infortunatum ON SOME VEGETABLE SEEDS WITH THEIR CHEMICAL INVESTIGATION

Abstract

An experiment was conducted on bioassay of different extracts of leaves of Clerodendrum infortunatum for each vegetable crop such as, kolmishak, bhendi, and borboti with the attempt for chemical investigation on effective plant extract. The chloroform extract of C. infortunatum significantly increased and enhanced germination, growth of shoot length and root length of yard long bean and swamp cabbage seeds compared with control. But germination, growth of shoot length and root length of lady’s finger is increased and enhanced by control treatment. The chloroform extract of C. infortunatum showed the highest germination at 7 days after sowing for these vegetable crops. Increased and enhanced but the lowest germination percentage (12.00%) was in T; at 2 DAS in swamp cabbage. Highest germination percentage was in T2 at 7 DAS for long yard bean, swamp cabbage, lady’s finger whereas lowest germination percentage was in T, at 2 DAS in those vegetables. Shoot length of swamp cabbage at different days after sowing influenced significantly by the effects of different extract. At 11 Days after sowing (DAS) with the leaf extract of C. infortunatum showed the highest shoot length 5.867 cm while the lowest shoot length (0.9267 cm) was recorded in control. Root length of swamp cabbage seedling at different days after sowing was lowest at 5 DAS and showed an increasing trend up to 11 DAS. Incase of lady’s finger the germination percentage was highest found in T, At 5 DAS highest germination found in T, (36.00). Shoot length of ladies finger at different days after sowing influenced significantly by the effects of different leaf extract but highest result found on control. Root length of lady’s finger seedling at different days after sowing was enhanced by control treatment. TLC of chloroform extracts of C. infortunatum at hexane indicated that it contains five compounds. Separation of individual components and their structure determination by spectral study in progress which will be reported in due course.