IN VITRO PROPAGATION AND NUCLEUS SEED PRODUCTION EFFICIENCY OF BARI DEVELOPED CLONE POTATO VARIETIES

Abstract

An experiment was conducted at Tissue Culture Lab and Net House of Tuber Crops Research Centre, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur from April 2016 to February 2017 to investigate In vitro propagation and nucleus seed production efficiency of BARI developed clone potato varieties. In vitro shoots of potato varieties BARI Alu-35, BARI Alu-36, BARI Alu-37, BARI Alu-40, BARI Alu-46 and BARI Alu-62 were used as planting materials in this experiment. BARI Alu-7 was used as check variety. Significant difference were found among the growth parameters of in vitro plantlets of the potato varieties. The maximum number of leaves (14.80), length of shoot (99.98cm), weight of fresh shoot (1.66 mg) were observed in BARI Alu-36. Length of root of plantlets varied significantly among the varieties. The highest length of root (5.54 cm) was recorded in BARI Alu-36. The maximum number of primary root (17.00), number of secondary root (28.20) and fresh weight of root (0.359 mg) were observed in BARI Alu-36. BARI Alu-36 showed better performance on most of the morphological traits under in vitro condition. On the other hand, survival percentage and nucleus seed (G generation) production of potato have been calculated from virus free in vitro plantlets of potato at net house of TCRC, BARI during November 2016 to February 2017. Number of G seed tuber per plant 21.2, 16.0, 18.6, 19.4, 30.4, 37.4 and 52.4 were recorded in BARI Alu -7, BARI Alu -36, BARI Alu-37, BARI Alu-62, BARI Alu-40, BARI Alu-35, and BARI Alu-46 respectively. The maximum weight of G 1 1 seeds per plant were found from the variety BARI Alu-46 (358.0 g) followed by BARI Alu-7 (306g), BARI Alu -62 (256.6g), BARI Alu- 36 (194.2g), BARI Alu- 40 (182.4g), BARI Alu-35(142g) and BARI Alu37(113.4g).