Investigation of Thrombolytic Potential of Combined Approach on Methanolic Extracts from Rosa, Centella asiatica, and Swertia using In-Vitro Technique

Abstract

This study aimed to investigate the phytochemical composition and thrombolytic potential of three plant extracts: Centella asiatica leaves, Rosa petals, and Swertia bark. Phytochemical analysis was conducted using standard constituent tests to investigate the presence of variety of bioactive compounds, but more extensive research is needed to isolate and characterize specific compounds and evaluate their therapeutic potential. Thrombolytic activity was assessed by measuring the percentage inhibition of clot lysis in human blood. The results demonstrated that all three plant extracts exhibited a certain degree of thrombolytic activity with Rosa exhibited 35.67% (moderate) thrombolytic activity, while Swertia and centella asiatica demonstrated 30.20% and 19.71% (mild) thrombolytic properties respectively. Furthermore, a combination of Centella asiatica leaves, Rosa petals, and Swertia bark extract was evaluated for its thrombolytic efficacy. While the combination exhibited some activity in breaking down blood clots, it did not consistently outperform the standard thrombolytic agent. The highest clot lysis observed was 27.76%, which, although significant, was lower than the 72% lysis achieved by the standard. Despite these findings, the study highlights the potential of these plant extracts as promising sources of natural thrombolytic agents. Additional examination is needed to fully elucidate the underlying mode of working, optimize extract preparation methods, and evaluate their safety and efficacy in clinical trials. Ultimately, these findings could contribute to the development of novel, natural-based therapies for thrombotic disorders.