IN VITRO PLANTLET REGENERATION IN MINT (Mentha spicata)

Abstract

The present study was undertaken in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-eBangla

Nagar, Dhaka-1207, during the period from June, 2017 to January, 2018 to evaluate the effect of different plant growth regulators (BA, IBA) on in vitro plantlet regeneration in Mint. The shoot tips of Mint were used as explants which were inoculated in MS media supplemented with different conc. (1.0, 1.5, 2.0 and 2.5 mg/l) of Benzyl adenine (BA) and Indole-3-butyric acid (IBA) (0.5, 1.0, 1.5 and 2.0 mg/l) either alone or in combination of both. The experiments were arranged in Completely Randomized Design (CRD) with three replications. The maximum shoot induction was obtained with 2.0 mg/L BA. The highest shoot length (12.12 cm) was found in 2.0 mg/L BA and minimum shoot length (2.90 cm) in control treatment. The highest percentage (85.00%) of root induction was recorded with BA 2.0 mg/l + IBA 1.0 mg/l in minimum (11.0 days). The highest length of root (3.25, 5.00 and 7.50 cm) at 21, 28 and 42 DAI respectively was found in 1.0 mg/l IBA and the control treatment found the lowest length of root at all DAI. The treatment BA 2.0 mg/l + IBA 1.0 mg/l gave the highest number of root (10, 15 and 20.67) at 21, 28 and 42 DAI respectively. Regenerated plantlets showed 90% survival during in growth chamber and 83.33% in shade house and 80 % in open atmosphere at direct sunlight. Therefore, an efficient protocol has been developed for in vitro regeneration of Mint which has great commercial value for year round production in Bangladesh.