Isolation of E.coli from Commercial Broiler Chickens and It's Histopathology

Abstract

Avian colibacillosis is considered to be a major bacterial disease in the poultry industry worldwide. The prevalence of colibacillosis in broiler chickens was studied from January to February 2017 that was presented at Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University. 50 swabs from liver, lungs, cloaca of 30 dead birds were collected in sterile nutrient broth as well as 30 liver samples for histopathology. Bacteria were isolated through cultural properties in MacConkey and Eosin Methylene Blue agar. Tissue samples were studied under light microscope after routine Hematoxylline and Eosin staining. Cloudy thickened air sacs, pericarditis, congestion in the liver, lung and spleen were observed in dead birds. On histopathological examination heterophils, lymphocytes, macrophages and fatty liver change found in liver. Based on cultural properties 31 (62%) isolates were found positive. All isolates of Boalkhali were found positive in bacteriological culture technique and no isolates from Betbunia was found positive.