N VITRO PLANT REGENERATION FROM INTERNODE AND LEAF SEGMENTS DERIVED CALLUS IN Adhatoda vasica NEES.

Abstract

Ile investigation was carried out 10 observe callus induction and subsequent regeneration potentiality of Adhatoda vasica NEES from internode and leaf segments of mature plants. The nature of callus was hard in texture and greenish white in colour in all concentrations of 2,4·D. On the other hand, the nature of callus was loose in texture and creamy in colour in all concentrations of NAA which was achieved from internode and leaf segments on MS medium supplemented with 4.0 mg/l NAA within four weeks of inoculation. The callus produced large number of shoots when cultured on MS medium with 1.0 mg/l BAP +0.1 mg/l NAA within 4 weeks of subculture. In vitro raised shoots were rooted on MS medium supplemented with 1.0 mg/l IBA within 4 weeks of subculture. III vitro grown plantlets with well root system were successfully established in natural condition through successive phages of acclimatization. The regenerated plants were healthy, uniform and identical to the donor plants and the survival percentage was 80.