Scientific exploration of Cytotoxic, Anti-Oxidant and Thrombolytic Activity of methanolic extract of Cassia Fistula (Leaves) and Cicer arietinum (Fruits) Remedies

Abstract

The investigation looked into the scientific underpinnings of the plants' potential medical uses, including their cytotoxic, thrombolytic, and antioxidant properties. Both the chickpea remedy and the Cassia fistula remedy showed moderate cytotoxic and notable thrombolytic and antioxidant activities, according to the study, indicating that they might be utilized as possible natural medication sources for the treatment of a range of illnesses. Objectives: To assess the possible chemical components and assess the effects on thrombolysis, antioxidants, and cytotoxicity. Materials & Methods: Methanol was used to extract the plant material from Cassia fistula and Cicer arietinum for the phytochemical testing, which were conducted in a lab. The thrombolytic test was conducted, and the coat lysis method was used to carry out the thrombolytic activity. The Brine Shrimp method was used to measure cytotoxic activity, while the free radical scavenging method was used to screen for antioxidants. Results: Numerous chemical components, including alkaloids, carbohydrates, tannins, glycosides, and saponins, were found in the extract of Cassia fistula and Cicer arietinum, according to phytochemical study. When the output of thrombolytic activity from these plant remedies is compared to the usual value of streptokinase (75.29%, Previous report), it is 39.35%. Additionally displayed is the antioxidant activity (IC50=5.56), which is contrasted with the standard ascorbic acid (IC50=9.072). The methanolic extract of Cassia fistula exhibits cytotoxic effect because its LC50 value is almost the same as that of vincristine sulfate. Conclusion: The plant exhibits phytochemical and pharmacological activities (thrombolytic, antioxidant, and cytotoxic) according to the data gathered.