STUDY ON THE EFFECTIVENESS OF DIFFERENT PRESERVATIVES ON THE PRESERVATION AND FIXATION OF PHYTOPLANKTON (Tetraselmis sp.) CELL

Abstract

A study was conducted on the effectiveness of different preservatives such as Formaldehyde, Ethyl alcohol and glycerin, Logul’s solution (neutral), Logul’s solution (acidic), Glutaraldehyde, and Transeu solutions on the preservation of phytoplankton (Tetraselmis sp.) cells. The main goal was to find appropriate chemical preservatives for preserving phytoplankton samples in laboratory conditions and determining the extent of damage caused by preservatives during long-term storage cells of phytoplankton. Tetraselmis sp. served as the experiment's target species. The Chlorodendrophyceae family includes it under the name marine phytoplankton. The Marine Fisheries and Technology Station, BFRI, Cox's Bazar provided the isolated Tetraselmis sp. sample (one liter of concentrated sample water). This sample was obtained by the BFRI in Bangladesh from the Bay of Bengal. The result showed among different preservatives Lugol’s solution (acidic) was the most effective preservative, whereas Glutaraldehyde was determined to be the least effective and the highest mean intact cell was found at formaldehyde and the lowest mean intact cells was found at Glutaraldehyde. This study would help to provide knowledge for choosing appropriate preservation during laboratory experiments and help to find the more suitable preservatives.