“In Vitro Pharmacological Investigations on Dichloromethane (DCM) Fraction of Sygyzium Samarangense Leaf Extract”

Abstract

The study was designed for pharmacological investigation of dichloromethane (DCM) fraction of methanol extract of the leaves of Syzygiumsamarangense(Family: Myrtaceae). The powdered leaves of Syzygiumsamarangensewere extracted with methanol and then partitioned with nhexane, dichloromethane and ethyl acetate consecutively. The DCM fraction remaining was investigated for total flavonoid content, total phenol content,brine shrimp lethality test and antimicrobial test. The fraction contained 21.073 mg AAE/gm of dried extract in total phenolic content assay and 0.581mg quercetin/gm of dried extract in total flavaniod content assay. Screening for cytotoxic properties using brine shrimp lethality bioassay with tamoxifen (LC50 value of 13.38μg/ml) as positive control showed that the fraction have considerable cytotoxic potency exhibiting LC50 value 2.00 μg/ml. In antimicrobial activity investigation, the DCM fraction showed low to moderate antibacterial and antifungal activity against the tested organisms compared to ciprofloxacin that was used as positive control. The DCM fraction showed strong cytotoxic activity, low antioxidant activity and low to moderate antimicrobial activity. Further investigations are needed for the proper identification and isolation of these bioactive compounds to produce safer drugs for treatment of harmful diseases