EVALUATION OF TRICHODERMA HARZIANUM TO CONTROL SCLEROTIUM ROLFSII AND FUSARIUM OXYSPORUM f. sp. LYCOPERSICI CAUSING DAMPING-OFF AND FOOT ROT OF TOMATO

Abstract

The research work with five set of experiments were conducted to evaluate the efficacy of T. harzianumin controlling S. rolfsii and F. oxysporum causing Damping off and Foot rot of tomato at Plant Pathology Laboratory of Hajee Mohammad Danesh Science and Technology (HSTU), Dinajpur, Bangladesh during the period of October, 2015 to December, 2016. The first experiment was isolating pathogens (Sclerotium rolfsii and Fusarium oxysporum). Isolated pathogens were identified as S. rolfsii and F. oxysporum based on morphological characters. The second experiment was evaluation of T. harzianum as biological control agent against S. rolfsii and F. oxysporum. Using the Stock culture of Trichoderma showed more than 60% inhibition of the radial growth of the test pathogen S. rolfsii over control. Trichoderma harzianum inhibited 80% radial mycial growth of Fusarium oxysporum after five days of inoculation. F. oxysporum. The rest of the experiments revealed that T4 which contains the first experiment (S. rolfsii formulation @20g/kg of soil + T. harzianum formulation @20g/kg of soil + T. harzianum suspension 2.45×107 CFU/ml), the second experiment (F. oxysporum formulation @20g/kg of soil + T. harzianum formulation @20g/kg of soil + T. harzianum suspension 2.45×107 CFU/ml) and the third experiment (S. rolfsii formulation @20g/kg of soil + F. oxysporum formulation @20g/kg of soil + T. harzianum formulation @20g/kg of soil + T. harzianum suspension 2.45×107 CFU/ml), showed minimum foot rot and damping off among all the treatments.