IN VITRO PROPAGATION OF BETEL VINE

Abstract

The experiment was carried out on Betel vine (Piper belle L.) in Biotechnology Lab. of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e Bangla Nagar, Dhaka-1 207 from the period of July 2014 to April 2015 to investigate the effect of BA, KIN, IAA and IDA on shoot proliferation, root formation and micropropagation protocol development. Nodal segment was used as explant. The highest percent (70%) of shoot proliferation and minimum days to shoot induction (21.67 days) were achieved on MS medium containing BA 1.0 mg/L + KIN 3.0 mg/L. Maximum average length of shoot (8.43 cm) was reported from media containing BA 0.5mg/L + KIN 4.0 mg/L. The highest percent (90%) of rooting and maximum 14.40 root/explant were reported in media treated with 3.0 mg/L IRA. Maximum 90% survival was observed in growth chamber and 83% during shade house and 64% in open atmosphere. A protocol for micropropagation of Betel vine, has been established and this experiment can be a useful tool for micropropagation of Betel vine.