OCCURENCE AND INCIDENCE OF MAJOR POTATO VIRUSES IN BANGLADESH AND THEIR DETECTION THROUGH ELISA TEST

Abstract

This study was conducted to reveal the incidence of major potato viruses collected from the main growing field of selected district in Bangladesh. Incidence of major potato viruses in eight major potato growing areas of Bangladesh, viz. Panchagarh, Thakurgaon, Nilphamari, Rangpur, Bogra, Chapainawabgonj, Pabna and Munshiganj were investigated in field samples. Overall, three upazilla (sub-districts) from each district (which were at least 20 km apart) were selected for samples collection and disease incidence monitoring. A total of 240 samples were collected on the basis of virus and viral like symptoms. Collected samples were stored at 4 0 C and analyzed at Potato Research Centre, BARI, Debigonj, Panchogorh and in Molecular Plant Virology lab, Central Research Laboratory, Sher-e-Bangla Agricultural University (SAU), Dhaka. Enzyme Linked Immunosorbent Assay (ELISA) tests revealed that Potato leaf roll virus (PLRV) was most predominant virus followed by Potato virus Y (PVY) and Potato virus X (PVX). During the year 2015-16, the average incidence of potato virus diseases in eight districts was about 48%. Single, double and triple, infections were 33.0, 47.3 and 2.0% respectively. The presence of the virus in the field of the main growing areas, as this study reported, is an alarming and need action to prevent spreading. This result also helps in taking management approaches for controlling viral disease in Bangladesh.