In Vitro Pharmacological Investigations on Aqueous Fraction of Phyllanthus acidus Leaf Extract

Abstract

The purpose of the study was to evaluate the cytotoxic, antioxidant and antimicrobial activity of aqueous fraction of Phyllunthus acidus leaf (Family:Phyllunthaceae) extract.The powder of leaf were extracted with methanol and then partitioned with n-hexane, DCM, and ethyl acetate, aqueous fraction was taken for experiment. The aqueous fraction was used to evaluate cytotoxic, antioxidant and antimicrobial activities. The cytotoxic activity was measured by brine shrimp lethality bioassay. LC50 value of aqueous fraction of Phyllanthus acidus was 2μg/ml in brine shrimp lethality test. The fraction contained 4.5mg AAE/g of total phenolic content and 4.868 mg AAE/g of total flavonoid content. The results of study clearly indicate the presence of cytotoxic and poor antioxidant properties of aqueous extract. The obtained results provide a support for the use of this plant in traditional medicine and its further investigation