Browsing by Author "Mannan, M. A."

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    DETECTION OF Escherichia coli AND Salmonella SPECIES FROM VEGETABLES SALAD IN DHAKA CITY
    (Sher-e-Bangla Agricultural University Research System (SAURES), 2023-07) Mannan, M. A.; Rahman, S.; Adnan, R.; Saud, B.; Chowdhury, S.
    The vegetable salad is a popular and healthy food for human beings. It may be contaminated by a lot of microbes. This research work was conducted to isolate and identify the Escherichia coli and Salmonella bacteria from mixed vegetable salad in Dhaka City, Bangladesh from 2020 to 2021. A total number of 120 mixed vegetable salad samples were collected from different restaurants, food corners and street vendors. Bacteria were identified based on cultural, staining and biochemical properties. Total viable count (TVC) and total coliform count (TCC) were determined. The mean ±SD values of TVC (log10CFU/gm) were 6.07±0.69, 5.42±0.69 and 7.04±0.48 for restaurant, food corner and street vendor, respectively. The TCC (log10CFU/gm) were 6.20±0.59, 5.23±0.59 and 6.70±0.57 in restaurant, food corner and street vendor samples, respectively. The highest contaminations of E. coli and Salmonella spp. were observed in street vendor salads which were 15% and 7.5%, respectively. The antimicrobial sensitivity test showed resistance to ampicillin, amoxicillin and tetracycline, while sensitive to ceftriaxone, gentamicin and streptomycin for both types of bacteria. So, these bacteria are zoonotic and the salad from different food shops should be prepared hygienically prior to consumption.
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    OCCURRENCE AND ANTIBIOTIC SENSITIVITY PROFILING OF Staphylococcus aureus ISOLATED FROM RAW CHEVON
    (Sher-e-Bangia Agricultural University Research System (SAURES), 2023-07) Hasnain, N.; Khatun, A.; Islam, M.; Mannan, M. A.
    The chevon is an animal source protein that is delicious, nutritious and a very well-known food for all. This study aimed to determine the prevalence as well as the antibiotic sensitivity of Staphylococcus aureus in chevon sold at the different wet markets in Dhaka City. A total of 120 samples of raw chevon were 4* collected from some selected areas. The cultural characteristics, staining and biochemical properties were used to isolate and identify the S. aureus bacteria. The highest and lowest Total Viable Count (TVC) in the supplied sample were log 10 9.22 CFU/gm and log 9.04 CFU/gm, respectively. The overall prevalence of S. aureus in Dhaka City was 47.5%. The highest prevalence was recorded at BNP Bazar (65%) of the Dhaka City. Isolates were investigated for antibiotic sensitivity profile using a Kirby-Bauer disc diffusion assay against five common antibiotics used in goats. The S. aureus showed the highest sensitivity to gentamicin (57.9%), followed by ciprofloxacin (56.14%), and the highest resistance pattern was shown against amoxicillin (100%), followed by ampicillin (92.98%), and tetracycline (63.15%). These bacterial contaminations in the chevon may be mitigated following proper hygienic management, proper boiling or cooking, and public health awareness.
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