Browsing by Author "Khan, Md. Maruf"

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Antioxidant, analgesic and toxic potentiality of stephania japonica (Thunb.) miers. leaf
(© 2011 Asian Network for Scientific Information., 2011) Rahman, Md Habibur; Alam, Md. Badrul; Chowdhury, N.S.; Kumar Jha, Mithilesh; M, Hasan; Khan, Md. Maruf; Rahman, Md. Saifur; Haque, M. Ekramul
In the present study crude methanolic extract of Stephania japonica leaf was investigated for possible antioxidant, analgesic and toxic activity. The extract showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging activity and reducing power assays. In both DPPH radical and NO scavenging assay, the extract exhibited moderate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 105.55±1.06 and 129.12±0.15 ug mL-1, respectively while the IC50 values of ascorbic acid were 12.30±0.11 and 18.64±0.22 ug mL-1, respectively. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid-induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg-1 b. wt. doses displayed significant (p<0.001) reduction in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg-1 b.wt. which is comparable to the standard, diclofenac sodium (86.52%). The extract was also investigated for toxic potentiality using Brine Shrimp lethality bioassay. In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with the LC50 value of 25.19±0.98 ug mL-1. The study clearly indicates that the extract possesses good analgesic and cytotoxic activity along with moderate antioxidant potential.
Antioxidant, analgesic and toxic potentiality of stephania japonica (Thunb.) miers. leaf
(© 2011 Asian Network for Scientific Information., 2011) Rahman, Md Habibur; Alam, Md. Badrul; Chowdhury, N.S.; Kumar Jha, Mithilesh; M, Hasan; Khan, Md. Maruf; Rahman, Md. Saifur; Haque, M. Ekramul
In the present study crude methanolic extract of Stephania japonica leaf was investigated for possible antioxidant, analgesic and toxic activity. The extract showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging activity and reducing power assays. In both DPPH radical and NO scavenging assay, the extract exhibited moderate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 105.55±1.06 and 129.12±0.15 ug mL-1, respectively while the IC50 values of ascorbic acid were 12.30±0.11 and 18.64±0.22 ug mL-1, respectively. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid-induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg-1 b. wt. doses displayed significant (p<0.001) reduction in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg-1 b.wt. which is comparable to the standard, diclofenac sodium (86.52%). The extract was also investigated for toxic potentiality using Brine Shrimp lethality bioassay. In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with the LC50 value of 25.19±0.98 ug mL-1. The study clearly indicates that the extract possesses good analgesic and cytotoxic activity along with moderate antioxidant potential.
Thank you, a good research antioxidant, antimicrobial and toxicity studies of the different fractions of fruits of Terminalia belerica Roxb
(© IDOSI Publications, 2011., 2011) Alam, Md. Badrul; Zahan, Ronok; Hasan, Mahedi; Maruf, M; Khan, Md. Maruf; S Rahman, Nargis; Chowdhury, M; Haque, Ekramul
The crude methanolic extract of the fruits of Terminalia belerica Roxb. along with its various organic fractions were tested to evaluate the toxicity, antioxidant (in vitro and in vivo) and antibacterial activity. The different assay methods, including total antioxidant activity, scavenging free radical, authentic peroxynitrite and reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. While antimicrobial activities were evaluated using agar disc diffusion method and measuring their MIC value. The EtOAc fraction, showed strong activity in all the model systems tested and in peroxynitrite model this fraction (IC50= 1.29±0.18 μg/ml) exerted four-fold stronger activity than standard penicillamine (IC50 value of 5.20±0.32 μg/ml). The reducing power of the extract was found to be concentration dependent. 50 The administration of the extract/fractions at a dose of 200 and 400 mg/kg body weight to the male Wistar rats increased the percentage inhibition of reduced glutathione, superoxide dismutase and catalase significantly. Whereas, lipid peroxidation level in hepatotoxic rats markedly decreased at a dose of 400 mg/kg body weight after 7 days. EtOAc fractions showed also the highest antibacterial effect against Staphylococcus aureus with zone of inhibition 30.00±0.25 mm. The Brine shrimp lethality bioassay was used to determine the toxicity of the extracts where dichloromethane (CH2 Cl2) fraction showed highest activity (LC50= 39.02±1.16 μg/ml). In conclusion, the study clearly indicated that the extract/fractions of Terminalia belerica Roxb possesses good antioxidant and antimicrobial activity along with moderate toxicity.
Thank you, a good research antioxidant, antimicrobial and toxicity studies of the different fractions of fruits of Terminalia belerica Roxb
(© IDOSI Publications, 2011., 2011) Alam, Md. Badrul; Zahan, Ronok; Hasan, Mahedi; Maruf, M; Khan, Md. Maruf; S Rahman, Nargis; Chowdhury, M; Haque, Ekramul
The crude methanolic extract of the fruits of Terminalia belerica Roxb. along with its various organic fractions were tested to evaluate the toxicity, antioxidant (in vitro and in vivo) and antibacterial activity. The different assay methods, including total antioxidant activity, scavenging free radical, authentic peroxynitrite and reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. While antimicrobial activities were evaluated using agar disc diffusion method and measuring their MIC value. The EtOAc fraction, showed strong activity in all the model systems tested and in peroxynitrite model this fraction (IC50= 1.29±0.18 μg/ml) exerted four-fold stronger activity than standard penicillamine (IC50 value of 5.20±0.32 μg/ml). The reducing power of the extract was found to be concentration dependent. 50 The administration of the extract/fractions at a dose of 200 and 400 mg/kg body weight to the male Wistar rats increased the percentage inhibition of reduced glutathione, superoxide dismutase and catalase significantly. Whereas, lipid peroxidation level in hepatotoxic rats markedly decreased at a dose of 400 mg/kg body weight after 7 days. EtOAc fractions showed also the highest antibacterial effect against Staphylococcus aureus with zone of inhibition 30.00±0.25 mm. The Brine shrimp lethality bioassay was used to determine the toxicity of the extracts where dichloromethane (CH2 Cl2) fraction showed highest activity (LC50= 39.02±1.16 μg/ml). In conclusion, the study clearly indicated that the extract/fractions of Terminalia belerica Roxb possesses good antioxidant and antimicrobial activity along with moderate toxicity.