Browsing by Author "HASAN, MAHMUDUL"

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GENETIC VARIABILITY AND CHARACTER ASSOCIATION OF SOYBEAN (Glycine max L. Merr.)
(DEPARTMENT OF GENETICS AND PLANT BREEDING, SHER-E-BANGLA AGRICULTURAL UNIVERSITY, DHAKA, BANGLADESH, 2018) HASAN, MAHMUDUL
The present research work was conducted to study the genetic diversity analysis of soybean during the period from December 2017 to May 2018 in rabi season in the experimental field of Sher-e-Bangla Agricultural University, Sher-eBangla Nagar, Dhaka. In this experiment, 22 soybean genotypes were used as experimental materials. Mean performance, variability, correlation, path analysis and genetic diversity analysis on different yield attributes and yield of soybean genotypes was estimated. Analysis of variance for each trait showed significant differences among the genotypes except pod wide, seed per pod, seed yield per plot and seed yield per hectare. In correlation study, highly significant positive association was recorded for seed yield of soybean genotypes with first flowering, days to 50% flowering, days to physiological, days to harvest maturity, plant height, number of branch per plant, number of pod per plant, seed yield per plant and yield per plot. Path coefficient analysis evidenced that 50% flowering, day to physiological maturity, plant height, number of branch per plant, pod per plant, 100 seed weight and yield per plot had the positive direct effect on yield per plant. Therefore, importance has to be given for these characters in the further breeding program to improve soybean yield. Multivariate analysis based on fifteen characters of twenty-two soybean genotypes was divided into three distant clusters. The maximum contribution of traits towards diversity was observed by first flowering, days to 50% flowering, days to physical maturity, days to harvest maturity, plant height, number of main branches per plant and pod length. As a result, these traits could be emphasized during the selection of parents for hybridization. The highest inter-cluster distance was observed between cluster I and II and the maximum intra cluster distance was found in cluster I. Considering group distance and other agro-morphogenic performance, genotypes G2 (GM0009) and G13 (GM0014) found the potential for future hybridization program in the response of increase soybean yield.
Isolation and Characterization of Multiple Drug Resistant Bacteria from Waste Water of Hospital and Non-Hospital Environment
(HAJEE MOHAMMOD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR, 2017-12) HASAN, MAHMUDUL; Professor Dr. Md. Khaled Hossain
A huge amount of antibiotics are normally used in hospital for patient care and disinfection which potentially raising antibiotic resistant bacteria in the liquid hospital waste that can also transmitted to non-hospital environments through drainage system. The current study was aimed to isolate and characterize multiple drug resistant bacteria from waste water of hospital and non-hospital environments. For this purpose the present research work was conducted during the period of (July-December) 2017 from untreated hospital and non-hospital waste water of different areas of Dinajpur district of Bangladesh. A total 20 samples were randomly collected and analyzed through different bacteriological, biochemical, molecular and antibiotic susceptibility testing. All 20 sample were 100% positive with one or more isolates, total 55 bacterial isolates were isolated, among them 32(58.2%) were from hospital environment and 23(42.1%) were from nonhospital environment. Result of total viable count showed that maximum countable bacteria (2.20×1010 ) CFUs/ml that were from MARMCH Site-2 and minimum number of countable bacteria (1.0×1010 ) CFUs/ml was isolated from sample of Kalitola. Among the isolates E.coli 16(29%), Pseudomonas spp 12(21.8%), Klebsiella spp 9(16.4%), Salmonella spp 8(14.5%), Staphylococcus spp 5(9%) and Vibrio spp 5(9%) were identified. Multi drug resistant (MDR) Pseudomonas aeruginosa was characterized from hospital waste water by 16s rRNA sequencing. The antibiotic sensitivity study revealed that among the hospital isolates, about (83.3%) were resistant against Ampicillin, followed by Amikacin, Kanamycin and Penicillin (77.8%). On the other hand, non- hospital isolates were resistant against Amoxicillin and Penicillin (66.7%) followed by Ampicillin and Vancomycin (58.3%). Both hospital and non-hospital isolates were sensitive to Gentamycin respectively 72.5% and 75%. The findings of the experiment clearly suggested that hospital waste water contained more MDR bacteria than non-hospital waste water which are released into receiving water bodies that may cause serious threat to public health. Reducing indiscriminate use of antibiotics in both hospital and non-hospital settings and use of waste water treatment plant (WTP) in hospital may reduce this problem.