Browsing by Author "Bacchu, M.S."

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Development of an Advanced DNA Biosensor for Pathogenic Vibrio Cholerae Detection in Real Sample
(Biosensors and Bioelectronics, Elsevier, 2021) Ali, M. R.; Bacchu, M.S.; Setu, M.A.A.; Akter, S.; Hasan, M. N.; Chowdhury, F.T.; Rahman, M.M.; Ahommed, M.S.; Khan, M.Z.H.
Due to the epidemics of emerging microbial diseases worldwide, the accurate and rapid quantification of pathogenic bacteria is extremely critical. In this work, a highly sensitive DNA-based electrochemical biosensor has been developed to detect Vibrio cholerae using gold nanocube and 3-aminopropyltriethoxysilane (APTES) modified glassy carbon electrode (GCE) with DNA carrier matrix. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) experiments were performed to interrogate the proposed sensor at each stage of preparation. The biosensor has demonstrated high sensitivity with a wide linear response range to target DNA from 10−8 to 10−14 (R2 = 0.992) and 10−14 to 10−27 molL−1 (R2 = 0.993) with a limit of detection (LOD) value of 7.41 × 10−30 molL−1 (S/N = 5). The biosensor also exhibits a selective detection behavior in bacterial cultures that belong to the same and distant genera. Moreover, the proposed sensor can be used for six consecutive DNA assays with a repeatability relative standard deviations (RSD) value of 5% (n = 5). Besides, the DNA biosensor shows excellent recovery for detecting V. cholerae in poultry feces, indicating that the designed biosensor could become a powerful tool for pathogenic microorganisms screening in clinical diagnostics, food safety, and environmental monitoring.
Development of an Advanced DNA Biosensor for Pathogenic Vibrio Cholerae Detection in Real Sample
(Biosensors and Bioelectronics, Elsevier, 2021-09-15) Ali, M.R.; Bacchu, M.S.; Setu, M.A.A.; Akter, S.; Hasan, M.N.; Chowdhury, F.T.; Rahman, M.M.; Ahommed, M.S.; Khan, M.Z.H.
Due to the epidemics of emerging microbial diseases worldwide, the accurate and rapid quantification of pathogenic bacteria is extremely critical. In this work, a highly sensitive DNA-based electrochemical biosensor has been developed to detect Vibrio cholerae using gold nanocube and 3-aminopropyltriethoxysilane (APTES) modified glassy carbon electrode (GCE) with DNA carrier matrix. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) experiments were performed to interrogate the proposed sensor at each stage of preparation. The biosensor has demonstrated high sensitivity with a wide linear response range to target DNA from 10−8 to 10−14 (R2 = 0.992) and 10−14 to 10−27 molL−1 (R2 = 0.993) with a limit of detection (LOD) value of 7.41 × 10−30 molL−1 (S/N = 5). The biosensor also exhibits a selective detection behavior in bacterial cultures that belong to the same and distant genera. Moreover, the proposed sensor can be used for six consecutive DNA assays with a repeatability relative standard deviations (RSD) value of 5% (n = 5). Besides, the DNA biosensor shows excellent recovery for detecting V. cholerae in poultry feces, indicating that the designed biosensor could become a powerful tool for pathogenic microorganisms screening in clinical diagnostics, food safety, and environmental monitoring.
Development of an Advanced DNA Biosensor for Pathogenic Vibrio Cholerae Detection in Real Sample
(Biosensors and Bioelectronics, Elsevier, 2021) Ali, M.R.; Bacchu, M.S.; Akter, S.; Hasan, M.N.; Chowdhury, F.T.; Rahman, M.M.; Ahommed, M.S.
Due to the epidemics of emerging microbial diseases worldwide, the accurate and rapid quantification of pathogenic bacteria is extremely critical. In this work, a highly sensitive DNA-based electrochemical biosensor has been developed to detect Vibrio cholerae using gold nanocube and 3-aminopropyltriethoxysilane (APTES) modified glassy carbon electrode (GCE) with DNA carrier matrix. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) experiments were performed to interrogate the proposed sensor at each stage of preparation. The biosensor has demonstrated high sensitivity with a wide linear response range to target DNA from 10−8 to 10−14 (R2 = 0.992) and 10−14 to 10−27 molL−1 (R2 = 0.993) with a limit of detection (LOD) value of 7.41 × 10−30 molL−1 (S/N = 5). The biosensor also exhibits a selective detection behavior in bacterial cultures that belong to the same and distant genera. Moreover, the proposed sensor can be used for six consecutive DNA assays with a repeatability relative standard deviations (RSD) value of 5% (n = 5). Besides, the DNA biosensor shows excellent recovery for detecting V. cholerae in poultry feces, indicating that the designed biosensor could become a powerful tool for pathogenic microorganisms screening in clinical diagnostics, food safety, and environmental monitoring.
Label Free Flexible Electrochemical DNA Biosensor for Selective Detection of Shigella flexneri in Real Food Samples
(Elsevier, 2023-02-01) Ali, M.R.; Bacchu, M.S.; Das, S.; S. Akter; Rahman, M.M.; Aly, M.Aly Saad; Khan, M.Z.H.
An effective tool for early-stage selective detection of the foodborne bacterial pathogen Shigella flexneri (S. flexneri) is essential for diagnosing infectious diseases and controlling outbreaks. Here, a label-free electrochemical DNA biosensor for monitoring S. flexneri is developed. To fabricate the biosensor, detection probe (capture probe) is immobilized on the surface of poly melamine (P-Mel) and poly glutamic acid (PGA), and disuccinimidyl suberate (DSS) functionalized flexible indium tin oxide (ITO) electrode. Anthraquinone-2-sulfonic acid monohydrate sodium salt (AQMS) is used as a signal indicator for the detection of S. flexneri. The proposed DNA biosensor exhibits a wide dynamic range with concentration of the targets ranging from 1 × 10−6 to 1 × 10−21 molL−1 with a limit of detection (LOD) of 7.4 × 10−22 molL−1 in the complementary linear target of S. flexneri, and a detection range of 8 × 1010–80 cells/ml with a LOD of 10 cells/ml in real S. flexneri sample. The proposed flexible biosensor provides high specificity for the detection of S. flexneri compared to other target signals such as discrete base mismatches and different bacterial species. The developed biosensor displayed excellent recoveries in detecting S. flexneri in spiked food samples. Therefore, the proposed biosensor can serve as a model methodology for the detection of other pathogens in a broad span of industries.